Variation of diazinon and amitraz susceptibility of Hyalomma marginatum (Acari: Ixodidae) in the Rabat-Sale-Kenitra region of Morocco.

In the present study, the acaricide susceptibility status of Hyalomma marginatum in Morocco was investigated in the Rabat-Sale-Kenitra region using the Larval Packet Test. The overall LC50 value for diazinon and amitraz was 115 ppm (95% CI: [104; 125]) and 22 ppm (95% CI: [21; 23]), respectively. The LC50 values varied significantly between the nine sampled locations (P<0.001) ranging from 75 ppm (95% CI: [65; 84]) in Ouelmes to 179 ppm (95% CI: [139; 201]) in Jorf El Melha for diazinon and from 18 ppm (95% CI: [15; 21]) in Skhirat to 28 ppm (95% CI: [24; 31]) in Ouelmes for amitraz. Sequencing of the target-site of diazinon, acetylcholinesterase 1 (AChE1), indicated that previously reported resistance mutations in AChE1 were absent in ticks from Jorf El Melha surviving 500 ppm diazinon. This study is the first report on the H. marginatum susceptibilty status to the most frequently used acaricides in Morocco and indicates that acaricide tick resistance is emerging.

Granulocytic anaplasmosis in 2 dogs from Quebec.

Two dogs from Quebec were diagnosed with granulocytic anaplasmosis. They both displayed fever, lethargy, and anorexia. Other clinical signs included vomiting, uveitis, polyarthritis, hepatomegaly, and splenomegaly. Thrombocytopenia, anemia, and lymphopenia were identified in both cases. Cytoplasmic inclusions were observed within neutrophils, and Anaplasma phagocytophilum infection was confirmed by polymerase chain reaction in both dogs.

Anaplasmose granulocytaire chez deux chiens au Québec. Deux chiens originaires du Québec ont été diagnostiqués avec une anaplasmose granulocytaire. Les chiens ont manifesté de façon aiguë de la fièvre, un abattement et de l'anorexie. D'autres signes cliniques ont été observés incluant vomissement, uvéite, polyarthrite, hépatomégalie et splénomagalie. Une thrombocytopénie, une anémie et une lymphopénie ont été détectées chez les deux chiens. Des inclusions intracytoplasmques étaient également présentent dans les neutrophiles et l'infection à Anaplasma phagocytophilum a été confirmée par réaction d'amplification en chaîne par la polymérase chez les deux chiens.(Traduit par les auteurs).